Genome-Wide Analysis of Alternative Splicing Events Responding to High Temperatures in Populus tomentosa Carr.

Through alternative splicing (AS) processes, eukaryotic genes can generate a variety of transcription isoforms that lower the expression levels normal transcripts or result in diversity genes’ activities. Then, AS plays significant role control plant development and stress tolerance. In this study, we analyzed Populus tomentosa Carr. TC1521’s episodes response to high temperatures. The samples treated at 25 °C, 30 35 40 °C produced total 10,418, 11,202, 9947, 14,121 events, respectively, which responded 4105, 4276, 4079, 4915 genes, representing 9.84%, 10.25%, 9.78%, 11.78% number transcribed respectively. most common pattern, accounting for 42.31% 51.00% all was intron retention (IR), followed by exon skipping (ES), accounted 9.14% 10.23% events. According sequence characterization, negatively correlated with guanine-cytosine content (GC content) but favorably length, number, gene level. Compared treatment 2001 distinct were discovered °C. They primarily enriched RNA degradation pathway valine, leucine, isoleucine route, according (gene ontology) GO Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis. These findings demonstrated how process might be severely impacted addition, information on helped us comprehend stress-resistance mechanisms new ways completed molecular design breeding.